Our research program is focused on understanding the role of the endothelial glycocalyx in regulating injury severity and outcomes in trauma patients. In combination with established animal models of trauma-hemorrhage and resuscitation (THR), we utilize custom-designed bioengineered cell culture systems to study the effect of mechanotransduction signaling on THR-related endotheliopathy, microvascular dysfunction and organ injury. Our research efforts are aimed at the identification of therapeutic strategies to prevent glycocalyx damage or restore loss of glycocalyx function caused by THR.